pRS421 (ATCC® 87475)

Applications: YE-type (episomal) shuttle vectorvector permitting visual detection of recombinants O-acetylhomoserine sulfhydrylase  /  Depositors: JD Boeke

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Designations pRS421
Depositors JD Boeke
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.2329998016357420
Vector: pRS421 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pRS401, YEp24
Construct size (kb): 6.232999801635742
Features: insert detection: lacZ'
marker(s): MET15
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
YE-type (episomal) shuttle vector
vector permitting visual detection of recombinants O-acetylhomoserine sulfhydrylase
Restriction digests of the clone give the following sizes (kb): BamHI--6.2; EcoRI--1.7, 4.5; XbaI--3.2, 2.0, 1.0.
met15 phenotype produces brown colonies when grown on Pb containing media.
MET15, MET17 and MET25 are synonymous.
This vector is useful for gene knockout experiments in hosts with a non-revertable met15 auxotrophic marker gene mutation.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Cost GJ, Boeke JD. A useful colony colour phenotype associated with the yeast selectable/counter-selectable marker MET15. Yeast 12: 939-941, 1996. PubMed: 8873447

Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801

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Shipped frozen
Shipping Information

 Frozen glycerol stock of Escherichia coli containing the plasmid.