pBBR1Tp (ATCC® 87448)

Applications: cloning vectormobilizable vectorvector permitting visual detection of recombinants  /  Depositors: DE Woods, D DeShazer

Designations pBBR1Tp
Permits and Restrictions

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Depositors DE Woods, D DeShazer
Biosafety Level 1
Distribution host: Escherichia coli DH5alpha
Vector Information
Size (kb): 5.314
Intact vector size: 5.314
Type of vector: plasmid
Cloning sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV PstI SmaI BamHI SpeI
XbaI BstXI SacII SacI
Polylinker sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI
SpeI XbaI BstXI SacII SacI
Construction: pBBR1MCS, tmpR
Host range: broad host range; Burkholderia pseudomallei; Burkholderia
cepacia; Escherichia coli
Features (with orientation and position when available):
coding sequence: MOB, <-, 5148-799
coding sequence: REP, ->, 1793-2455
MCS: SacI...KpnI, ->, 3220-3322
marker(s): tmpR, ->, 4387-4623
Vector: pBBR1Tp (plasmid)
Construction: pBBR1MCS, tmpR
Construct size (kb): 5.314
Features: marker(s): tmpR
MCS: SacI...KpnI
coding sequence: MOB
coding sequence: REP
cloning vector
mobilizable vector
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): PstI--5.4; EcoRI--4.0, 0.64, 0.8; PvuII--2.4, 1.35, 1.1, 0.4
Plasmid is compatible with multiple replicons and can be transferred to other gram-negative bacteria by conjugation when the RK2 transfer functions are provided in trans.
Broad host range cloning vector allowing blue/white color detection of recominants and carrying the trimethoprim resistance gene as a selectable marker.
Media ATCC® Medium 2031: LB + 1.5 mg/ml trimethoprim
Growth Conditions
Temperature: 37.0°C

DeShazer D, Woods DE. Broad-host-range cloning and cassette vectors based on the R388 trimethoprim resistance gene. BioTechniques 20: 762-764, 1996. PubMed: 8723912

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Shipping Information Distributed: freeze-dried