pBBR1Tp (ATCC® 87448)

Applications: cloning vectormobilizable vectorvector permitting visual detection of recombinants  /  Depositors: DE Woods, D DeShazer

Permits and Restrictions

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Designations pBBR1Tp
Depositors DE Woods, D DeShazer
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Distribution host: Escherichia coli DH5alpha
Vector Information
Size (kb): 5.314
Intact vector size: 5.314
Type of vector: plasmid
Cloning sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV PstI SmaI BamHI SpeI
XbaI BstXI SacII SacI
Polylinker sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI
SpeI XbaI BstXI SacII SacI
Construction: pBBR1MCS, tmpR
Host range: broad host range; Burkholderia pseudomallei; Burkholderia
cepacia; Escherichia coli
Features (with orientation and position when available):
coding sequence: MOB, <-, 5148-799
coding sequence: REP, ->, 1793-2455
MCS: SacI...KpnI, ->, 3220-3322
marker(s): tmpR, ->, 4387-4623
Vector: pBBR1Tp (plasmid)
Construction: pBBR1MCS, tmpR
Construct size (kb): 5.314
Features: marker(s): tmpR
MCS: SacI...KpnI
coding sequence: MOB
coding sequence: REP
cloning vector
mobilizable vector
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): PstI--5.4; EcoRI--4.0, 0.64, 0.8; PvuII--2.4, 1.35, 1.1, 0.4
Plasmid is compatible with multiple replicons and can be transferred to other gram-negative bacteria by conjugation when the RK2 transfer functions are provided in trans.
Broad host range cloning vector allowing blue/white color detection of recominants and carrying the trimethoprim resistance gene as a selectable marker.
Media ATCC® Medium 2031: LB + 1.5 mg/ml trimethoprim
Growth Conditions
Temperature: 37.0°C

DeShazer D, Woods DE. Broad-host-range cloning and cassette vectors based on the R388 trimethoprim resistance gene. BioTechniques 20: 762-764, 1996. PubMed: 8723912

Shipped frozen
Shipping Information Distributed: freeze-dried