pUC19Sce [pUCSce] (ATCC® 87437)

Applications: vector permitting visual detection of recombinants  /  Depositors: HP Schweizer

Permits and Restrictions

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Designations pUC19Sce [pUCSce]
Depositors HP Schweizer
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 2.6860001087188720
Vector: pUC19Sce (plasmid)
Construction: pUC19
Construct size (kb): 2.686000108718872
Features: marker(s): ampR
replicon: pMB1
restriction site: I-SceI
restriction site: SmaI
coding sequence: lacZalpha
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): SmaI--2.7; AvaI--2.7; BglI--1.6, 1.1.
Useful for rescuing cloned bacterial inserts by digestion with I-SceI, an enzyme recognizing a rare 18 bp sequence which has yet to be found in bacterial DNA.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Schweizer HP, Hoang TT. An improved system for gene replacement and xylE fusion analysis in Pseudomonas aeruginosa. Gene 158: 15-22, 1995. PubMed: 7789804

Shipped freeze-dried