p424 GPD (ATCC® 87357)

Applications: YX-type (expression) shuttle vector  /  Depositors: M Funk

Permits and Restrictions

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Designations p424 GPD
Depositors M Funk
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host Escherichia coli HB101 (ATCC 33694)
Vector Information
Intact vector size: 6.527
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS424
Host range: Saccharomyces cerevisiae; Candida robusta; Escherichia coli
Features (with orientation and position when available):
replicon: 2 micron, →
marker(s): TRP1, →
terminator: CYC1, →
promoter for expression: GPD, ←
marker(s): ampR, ←
Vector: p424 GPD (plasmid)
Promoters: Promoter for expression GPD
Construction: pRS424
Marker(s): TRP1, ampR
Construct size (kb): 6.527
TRP1, ampR
Promoter for expression: GPD Replicon: 2 micron
Terminator: CYC1
YX-type (expression) shuttle vector
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 1.7, 1.0, 0.68; EcoRI--6.5; BamHI--6.5.
High copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37 C

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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Component of: ATCC 87669
Shipped frozen