p414 GALS (ATCC® 87344)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

Designations p414 GALS
Depositors M Funk
Biosafety Level 1
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.4289999008178710
Intact vector size: 5.429
Type of vector: plasmid
Cloning sites: SpeI BamHI SmaI PstI EcoRI ClaI SalI XhoI
Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI
Construction: pRS414, GALS promoter
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): TRP1, ->, 200-1213
terminator: CYC1, ->, 1895-2155
MCS: XhoI...SpeI, ->, 2155-2212
promoter for expression: GALS, <-, 2219-2634
marker(s): ampR, <-, 3881-4811
centromere: CEN6/ARSH4, ->, 4915-5429
Vector: p414 GALS (plasmid)
Promoters: Promoter for expression GALS
Construction: pRS414, GALS promoter
Construct size (kb): 5.428999900817871
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: GALS
MCS: XhoI...SpeI
terminator: CYC1
centromere: CEN6/ARSH4
YX-type (expression) shuttle vector
vector with low copy number
Restriction digests of the clone give the following sizes (kb): BamHI--5.4; EcoRI--5.4; SacI/XbaI--3.2, 1.8, 0.41.
The galactokinase (GALS) promoter is a deletion variant of GAL1, which lacks one and a half of the 3 UAS elements required for full induction of the promoter by galactose.
The promoter is tightly repressed by glucose and is induced at a moderate level by galactose.
Low copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736

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