pUK (ATCC® 87209)

Applications: expression vector  /  Depositors: B Huang

Permits and Restrictions

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Designations pUK
Depositors B Huang
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 2.4300000667572020
Vector: pUK (plasmid)
Promoters: Promoter tac
Construction: pUC19, pKK223-3
Marker(s):ampR
Construct size (kb): 2.430000066757202
Features: marker(s): ampR
promoter: tac
replicon: pMB1
MCS: HindIII-EcoRI
ribosome-binding site: Shine-Dalgarno
transcription terminator: rrnB
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.4; BamHI--2.4; HindIII--2.4.
A small, high-copy-number vector suitable for both in vitro (using the coupled transcription/translation system of Zubay) and in vivo expression.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Huang B, et al. A small, high-copy-number vector suitable for both in vitro and in vivo gene expression. Gene 151: 143-145, 1994. PubMed: 7828863

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map