pKSM715 (ATCC® 87161)

Applications: expression vectorvector permitting RNA synthesis in vitrovector permitting production of single-stranded DNAvector permitting visual detection of recombinants  /  Depositors: K Ippen-Ihler, S Maneewannakul

Designations pKSM715
Permits and Restrictions

View Permits

Depositors K Ippen-Ihler, S Maneewannakul
Biosafety Level 1
Vector Information
Size (kb): 5.0409998893737790
Vector: pKSM715 (phagemid)
Promoters: Promoter for in vitro transcription T7lac
Construction: pKSM713, pETIIa
Construct size (kb): 5.040999889373779
Features: insert detection: lacZalpha
marker(s): kanR
promoter for expression: lacUV5
promoter for in vitro transcription: T7lac
replicon: fd
replicon: pMB1
repressor gene: lacI
MCS: BamHI...EcoRI
terminator: trpA
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB
expression vector
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI--5.0; PstI--4.3, 0.6.
Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglII--3.47; EcoRI--0.39; KpnI--0.36, 5.02; NcoI--0.33, 2.87; PstI--2.58, 3.25; PvuI--0.51; PvuII--0.48, 3.20, 3.56, 3.90, 3.99; SacI--0.38, 5.03; SalI--2.57.
Differs from pKSM713 (ATCC 87159) only in the substitution of lacI for lacIq.
Expression vector.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C

Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823

Shipped freeze-dried