pKSM712 (ATCC® 87158)

Applications: expression vectorvector permitting RNA synthesis in vitrovector permitting production of single-stranded DNAvector permitting visual detection of recombinants  /  Depositors: K Ippen-Ihler, S Maneewannakul

Designations pKSM712
Permits and Restrictions

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Depositors K Ippen-Ihler, S Maneewannakul
Biosafety Level 1
Vector Information
Size (kb): 3.7409999370574950
Vector: pKSM712 (phagemid)
Promoters: Promoter for in vitro transcription T7lac
Construction: pKSM710, pUC4-KIXX
Construct size (kb): 3.740999937057495
Features: insert detection: lacZalpha
marker(s): kanR
promoter for expression: lacUV5
promoter for in vitro transcription: T7lac
replicon: fd
replicon: pMB1
MCS: BamHI...EcoRI
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB
expression vector
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--3.75; EcoRI--3.75; PstI--3.75.
Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglII--2.89; EcoRI--0.39; NcoI--0.33, 3.49; PstI--3.11; PvuI--0.51; PvuII--0.48, 2.80, 3.16.
Differs from pKSM711 (ATCC 87157) only in the orientation of the kanamycin resistance gene.
Expression vector.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C

Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823

Shipped freeze-dried