pKSM710 (ATCC® 87156)

Applications: contains easily purifiable cassette(s) for constructionexpression vectorvector permitting RNA synthesis in vitrovector permitting production of single-stranded DNAvector permitting visual detection of recombinants  /  Depositors: K Ippen-Ihler, S Maneewannakul

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Designations pKSM710
Depositors K Ippen-Ihler, S Maneewannakul
Biosafety Level 1
Vector Information
Size (kb): 5.0710000991821290
Vector: pKSM710 (phagemid)
Promoters: Promoter for expression lambda PL
Construction: pSM702, pMAMPF3
Marker(s):ampR,cmlR (am)
Construct size (kb): 5.071000099182129
Features: insert detection: lacZalpha
marker(s): ampR
marker(s): cmlR (am)
promoter for expression: lacUV5
promoter for expression: lambda PL
promoter for in vitro transcription: T7lac
replicon: fd
replicon: pMB1
terminator: trpA
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB
Applications
contains easily purifiable cassette(s) for construction
expression vector
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI--4.5, 0.5; PstI--5.0, DraIII--3.0, 2.1; DraIII/PvuI--2.0, 1.6, 0.9, 0.6.
Vector contains the following restriction sites (approximate kb from nt 1): BamHI--4.43; BglI--1.87; BglII--4.09; EcoRI--4.03, 4.48; NcoI--4.42; PstI--2.00; PvuI--2.13, 4.60; PvuII--3.40, 4.57.
Expression vector containing a 2.57 kb BglII-BspHI cassette useful for constructing a variety of expression vectors.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823

Shipped freeze-dried
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