pKSM710 (ATCC® 87156)

Applications: contains easily purifiable cassette(s) for constructionexpression vectorvector permitting RNA synthesis in vitrovector permitting production of single-stranded DNAvector permitting visual detection of recombinants  /  Depositors: K Ippen-Ihler, S Maneewannakul

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Designations pKSM710
Depositors K Ippen-Ihler, S Maneewannakul
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.0710000991821290
Vector: pKSM710 (phagemid)
Promoters: Promoter for expression lambda PL
Construction: pSM702, pMAMPF3
Marker(s):ampR,cmlR (am)
Construct size (kb): 5.071000099182129
Features: insert detection: lacZalpha
marker(s): ampR
marker(s): cmlR (am)
promoter for expression: lacUV5
promoter for expression: lambda PL
promoter for in vitro transcription: T7lac
replicon: fd
replicon: pMB1
terminator: trpA
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB
contains easily purifiable cassette(s) for construction
expression vector
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI--4.5, 0.5; PstI--5.0, DraIII--3.0, 2.1; DraIII/PvuI--2.0, 1.6, 0.9, 0.6.
Vector contains the following restriction sites (approximate kb from nt 1): BamHI--4.43; BglI--1.87; BglII--4.09; EcoRI--4.03, 4.48; NcoI--4.42; PstI--2.00; PvuI--2.13, 4.60; PvuII--3.40, 4.57.
Expression vector containing a 2.57 kb BglII-BspHI cassette useful for constructing a variety of expression vectors.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823

Shipped freeze-dried