K Ippen-Ihler, S Maneewannakul
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Size (kb): 5.0710000991821290
Vector: pKSM710 (phagemid)
Promoters: Promoter for expression lambda PL
Construction: pSM702, pMAMPF3
Construct size (kb): 5.071000099182129
Features: insert detection: lacZalpha
marker(s): cmlR (am)
promoter for expression: lacUV5
promoter for expression: lambda PL
promoter for in vitro transcription: T7lac
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB
contains easily purifiable cassette(s) for construction
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI--4.5, 0.5; PstI--5.0, DraIII--3.0, 2.1; DraIII/PvuI--2.0, 1.6, 0.9, 0.6.
Vector contains the following restriction sites (approximate kb from nt 1): BamHI--4.43; BglI--1.87; BglII--4.09; EcoRI--4.03, 4.48; NcoI--4.42; PstI--2.00; PvuI--2.13, 4.60; PvuII--3.40, 4.57.
Expression vector containing a 2.57 kb BglII-BspHI cassette useful for constructing a variety of expression vectors.
Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823