pBSL175 (ATCC® 87150)

Applications: contains easily purifiable cassette(s) for construction omega-aadA1  /  Depositors: MF Alexeyev

Permits and Restrictions

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Designations pBSL175
Depositors MF Alexeyev
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.2519998550415040
Vector: pBSL175 (plasmid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pSU18
Construct size (kb): 4.251999855041504
Features: marker(s): cmlR
promoter for in vitro transcription: T7 (phi10)
replicon: p15A
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: aadA (spcR, strR)
contains easily purifiable cassette(s) for construction omega-aadA1
Restriction digests of the clone give the following sizes (kb): MluI--2.2, 2.1; EcoRI--2.2, 2.1; BamHI--2.2, 2.1.
Plasmid useful for vector construction. Contains an omega-Gm element (aadA, strR, spcR) flanked by multiple cloning sites duplicated in tandem orientation.
Media ATCC® Medium 1632: LB medium (ATCC medium 1065) with 10 mcg/ml chloramphenicol
Growth Conditions
Temperature: 37.0°C

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Shipped frozen