pBSL168 (ATCC® 87149)

Applications: contains easily purifiable cassette(s) for construction wild type beta-lactamase  /  Depositors: MF Alexeyev

Permits and Restrictions

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Designations pBSL168
Depositors MF Alexeyev
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Distribution host: Escherichia coli DH5
Vector Information
Size (kb): 3.3570001125335690
Name of vector: sBSL150
Intact vector size: 2.200
Type of vector: plasmid
Vector end: mLuI
Vector end: mLuI
Cloning sites:
Polylinker sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI
SpeI XbaI NotI/EagI BstXI SacII SacI mLuI
Construction: pSU18
Host range: Escherichia coli
Features (with orientation and position when available):
MCS: SacI...KpnI mLuI, ->
MCS: mLuI KpnI...SacI, ->
replicon: p15A
marker(s): cmLR, <-
Cross references:
Vector: pBSL168 (plasmid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pSU18
Construct size (kb): 3.357000112533569
Features: marker(s): cmlR
promoter for in vitro transcription: T7 (phi10)
replicon: p15A
MCS: KpnI...SacI MluI
MCS: SacI...KpnI MluI
coding sequence: bla (ampR)
contains easily purifiable cassette(s) for construction wild type beta-lactamase
Restriction digests of the clone give the following sizes (kb): BamHI--2.0, 1.35; EcoRI--2.05, 1.25; MluI--2.1, 1.15; SstI--1.9, 1.4; NcoI/BamHI--1.4, 1.1, 0.9; NotI/ScaI--1.1, 0.9, 0.8, 0.7.
Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA in vitro. Contains a beta-lactamase (ampR) cassette flanked by multiple cloning sites duplicated in inverted orientation.
The PstI and HincII sites in the bla gene have been removed.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Shipped freeze-dried
Shipping Information Distributed: freeze-dried