pBSL168 (ATCC® 87149)

Applications: contains easily purifiable cassette(s) for construction wild type beta-lactamase  /  Depositors: MF Alexeyev

Designations pBSL168
Permits and Restrictions

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Depositors MF Alexeyev
Biosafety Level 1
Distribution host: Escherichia coli DH5
Vector Information
Size (kb): 3.3570001125335690
Name of vector: sBSL150
Intact vector size: 2.200
Type of vector: plasmid
Vector end: mLuI
Vector end: mLuI
Cloning sites:
Polylinker sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI
SpeI XbaI NotI/EagI BstXI SacII SacI mLuI
Construction: pSU18
Host range: Escherichia coli
Features (with orientation and position when available):
MCS: SacI...KpnI mLuI, ->
MCS: mLuI KpnI...SacI, ->
replicon: p15A
marker(s): cmLR, <-
Cross references:
Vector: pBSL168 (plasmid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pSU18
Construct size (kb): 3.357000112533569
Features: marker(s): cmlR
promoter for in vitro transcription: T7 (phi10)
replicon: p15A
MCS: KpnI...SacI MluI
MCS: SacI...KpnI MluI
coding sequence: bla (ampR)
contains easily purifiable cassette(s) for construction wild type beta-lactamase
Restriction digests of the clone give the following sizes (kb): BamHI--2.0, 1.35; EcoRI--2.05, 1.25; MluI--2.1, 1.15; SstI--1.9, 1.4; NcoI/BamHI--1.4, 1.1, 0.9; NotI/ScaI--1.1, 0.9, 0.8, 0.7.
Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA in vitro. Contains a beta-lactamase (ampR) cassette flanked by multiple cloning sites duplicated in inverted orientation.
The PstI and HincII sites in the bla gene have been removed.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

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