Host
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Distribution host: Escherichia coli DH5
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Vector Information
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Size (kb): 4.6690001487731930 DESCRIPTION OF VECTOR COMPONENT: Name of vector: sBSL98 Intact vector size: 3.100 Type of vector: phagemid Vector end: mLuI Vector end: mLuI Cloning sites: Polylinker sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI NotI/EagI BstXI SacII SacI mLuI Construction: pBluescript Host range: Escherichia coli Features (with orientation and position when available): MCS: KpnI...SacI mLuI, -> MCS: mLuI KpnI...SacI, -> replicon: pMB1 marker(s): ampR, <- replicon: f1, <- Cross references: Vector: pBSL142 (phagemid) Promoters: Promoter for in vitro transcription f1T7 (phi10) Construction: pBluescript Marker(s):ampR,gtmR Construct size (kb): 4.669000148773193 Features: marker(s): ampR promoter for in vitro transcription: f1T7 (phi10) replicon: f1 replicon: pMB1 MCS: KpnI...SacI MluI MCS: MluI KpnI...SacI coding sequence: aacC1 (gtmR)
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Applications
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contains easily purifiable cassette(s) for construction omega - aacC1
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Comments
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Restriction digests of the clone give the following sizes (kb): BamHI--2.8, 1.6; EcoRI--2.95, 1.75; KpnI--2.8, 1.65; MluI--2.9, 1.6; SstI--2.8, 1.7. Plasmid useful for generation of marked deletions and insertions in cloned DNA in vitro. Contains an omega - Gm element (aacC1, gtmR) flanked by multiple cloning sites duplicated in tandem orientation.
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References
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Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718
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Shipping Information
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Distributed: freeze-dried
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