pBSL142 (ATCC® 87147)

Applications: contains easily purifiable cassette(s) for construction omega - aacC1  /  Depositors: MF Alexeyev

Permits and Restrictions

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Designations pBSL142
Depositors MF Alexeyev
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli DH5
Vector Information
Size (kb): 4.6690001487731930
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: sBSL98
Intact vector size: 3.100
Type of vector: phagemid
Vector end: mLuI
Vector end: mLuI
Cloning sites:
Polylinker sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI
SpeI XbaI NotI/EagI BstXI SacII SacI mLuI
Construction: pBluescript
Host range: Escherichia coli
Features (with orientation and position when available):
MCS: KpnI...SacI mLuI, ->
MCS: mLuI KpnI...SacI, ->
replicon: pMB1
marker(s): ampR, <-
replicon: f1, <-
Cross references:
Vector: pBSL142 (phagemid)
Promoters: Promoter for in vitro transcription f1T7 (phi10)
Construction: pBluescript
Marker(s):ampR,gtmR
Construct size (kb): 4.669000148773193
Features: marker(s): ampR
promoter for in vitro transcription: f1T7 (phi10)
replicon: f1
replicon: pMB1
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: aacC1 (gtmR)
Applications
contains easily purifiable cassette(s) for construction omega - aacC1
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.8, 1.6; EcoRI--2.95, 1.75; KpnI--2.8, 1.65; MluI--2.9, 1.6; SstI--2.8, 1.7.
Plasmid useful for generation of marked deletions and insertions in cloned DNA in vitro. Contains an omega - Gm element (aacC1, gtmR) flanked by multiple cloning sites duplicated in tandem orientation.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map