pTZ19RBS.K (ATCC® 87124)

Applications: vector permitting construction of fusion proteinsvector permitting positive selection for insertsvector permitting visual detection of recombinants  /  Depositors: O De Backer

Designations pTZ19RBS.K
Permits and Restrictions

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Depositors O De Backer
Biosafety Level 1
Vector Information
Size (kb): 2.8959999084472660
Vector: pTZ19RBS.K (phagemid)
Promoters: Promoter T7 (phi10)
Construction: pTZ19R
Construct size (kb): 2.895999908447266
Features: insert detection: lacZ'
marker(s): ampR
promoter: T7 (phi10)
promoter: lacOP
replicon: f1
replicon: pMB1
MCS: EcoRI...HindIII
vector permitting construction of fusion proteins
vector permitting positive selection for inserts
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--2.9, EcoRI--2.9, PstI--2.9.
Cloning into the BamHI site followed by transformation into an EcoK r+ m+ host, increases the relative transformation efficiency of recombinant molecules, provided that the cloned insert is free of non-methylated EcoK sites.
Vector allowing positive selection and blue/white color detection for cloned inserts.
Vector constructed from pTZ19R by elimination of the EcoK restriction site in the ampR region and creation of a new EcoK site within the polylinker, containing a BamHI site as the central hexamer of the EcoK recognition sequence.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

De Backer O, et al. Positive selection of recombinant plasmids based on the EcoK restriction activity of Escherichia coli K-12. Gene 150: 197-198, 1994. PubMed: 7959053

Shipped freeze-dried