pTZ19RBS.K (ATCC® 87124)

Applications: vector permitting construction of fusion proteinsvector permitting positive selection for insertsvector permitting visual detection of recombinants  /  Depositors: O De Backer

Permits and Restrictions

View Permits

Designations pTZ19RBS.K
Depositors O De Backer
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 2.8959999084472660
Vector: pTZ19RBS.K (phagemid)
Promoters: Promoter T7 (phi10)
Construction: pTZ19R
Construct size (kb): 2.895999908447266
Features: insert detection: lacZ'
marker(s): ampR
promoter: T7 (phi10)
promoter: lacOP
replicon: f1
replicon: pMB1
MCS: EcoRI...HindIII
vector permitting construction of fusion proteins
vector permitting positive selection for inserts
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--2.9, EcoRI--2.9, PstI--2.9.
Cloning into the BamHI site followed by transformation into an EcoK r+ m+ host, increases the relative transformation efficiency of recombinant molecules, provided that the cloned insert is free of non-methylated EcoK sites.
Vector allowing positive selection and blue/white color detection for cloned inserts.
Vector constructed from pTZ19R by elimination of the EcoK restriction site in the ampR region and creation of a new EcoK site within the polylinker, containing a BamHI site as the central hexamer of the EcoK recognition sequence.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

De Backer O, et al. Positive selection of recombinant plasmids based on the EcoK restriction activity of Escherichia coli K-12. Gene 150: 197-198, 1994. PubMed: 7959053

Shipped freeze-dried