pMR101 (ATCC® 87115)

Applications: expression vectorvector permitting production of single-stranded DNA  /  Depositors: L Regan, M Munson

Designations pMR101
Permits and Restrictions

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Depositors L Regan, M Munson
Biosafety Level 1
Distribution host: Escherichia coli JM101
Vector Information
Size (kb): 4.0999999046325680
Intact vector size: 4.100
Type of vector: phagemid
Cloning sites: NcoI BamHI
Polylinker sites:
Other unique sites: EcoNI, SalI, BstEII, PstI, BglII, XbaI
Construction: pTM201/NS3-3, pET8c, pACYC177, pET11d
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: M13, ->, 11-467
replicon: p15A, ->, 1585-1587
marker(s): kanR, ->, 2179-2994
operator: lac, <-, 3788-3804
promoter for expression: T7 (phi10), <-, 3808-3827
Vector: pMR101 (phagemid)
Promoters: Promoter for expression T7 (phi10)
Construction: pTM201/NS3-3, pET8c, pACYC177, pET11d
Construct size (kb): 4.099999904632568
Features: marker(s): kanR
operator: lac
promoter for expression: T7 (phi10)
replicon: M13
replicon: p15A
expression vector
vector permitting production of single-stranded DNA
Restriction digests of the clone give the following sizes (kb): BamHI--4.1; BstEII/PstI--3.0, 1.08; SalI--4.1.
There is an error in the reference in figures 1 and 2. The restriction sites, XbaI and BglII, are drawn in reverse order on the maps for pMR101.
Expression vector (T7-based) with a kanR marker and a P15A replicon compatible with ColE1-derived plasmids. Particularly useful for co-transformation with ColE1-based ampR T7 expression vectors and the production of two proteins in the same cell.
If used in an Escherichia coli strain that expresses T7 polymerase under the control of the lacUV5 promotor (such as BL21(DE3)), addition of IPTG can result in high levels of recombinant protein production.
Use of 5'NcoI and 3'BamHI cloning sites is similar to that of other expression systems, which facilitiates transfer of genes into these pMR vectors.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C

Munson M, et al. ColE1-compatible vectors for high-level expression of cloned DNAs from the T7 promoter. Gene 144: 59-62, 1994. PubMed: 8026759

Mary Munson, personal communication

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