pT7/Nde (ATCC® 87050)

Other IDs:

Nucleotide (GenBank) : U25273 Expression cloning vector pT7/Nco, complete sequence.

 /  Applications: expression vector  /  Depositors: RS Haun

Permits and Restrictions

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Designations pT7/Nde
Other IDs

Nucleotide (GenBank) : U25273 Expression cloning vector pT7/Nco, complete sequence.

Depositors RS Haun
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 2.9000000953674320
Vector: pT7/Nde (plasmid)
Promoters: Promoter SP6
Construction: pGEM-3, pET-3a
Construct size (kb): 2.900000095367432
Features: marker(s): ampR
promoter: SP6
promoter: T7 (phi10)
replicon: pMB1
MCS: HindIII...XbaI
ribosome-binding site: gene 10
expression vector
Restriction digests of the clone give the following sizes (kb): XbaI--2.90; HindIII--2.90; NdeI--2.90.
The NdeI site (CATATG) includes the initiator methionine codon. The NdeI cloning site can also be used in conjunction with the downstream BamHI site.
Differs from pT7/NcoI (ATCC 87051) only in the cloning site and reading frame for the insert.
Expression vector, maintained at high copy number.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Hong JX, et al. Effect of ADP-ribosylation factor amino-terminal deletions on its GTP-dependent stimulation of cholera toxin activity. J. Biol. Chem. 269: 9743-9745, 1994. PubMed: 8144566

Shipped freeze-dried