pCAT/LIC (ATCC® 87046)

Other IDs:

Nucleotide (GenBank) : U25266 Ligation-independent promoter-cloning vector pLUC/LIC, complete sequence.

 /  Applications: promoter-cloning vector  /  Depositors: RS Haun

Designations pCAT/LIC
Permits and Restrictions

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Depositors RS Haun
Other IDs

Nucleotide (GenBank) : U25266 Ligation-independent promoter-cloning vector pLUC/LIC, complete sequence.

Biosafety Level 1
Vector Information
Size (kb): 4.4000000953674320
Vector: pCAT/LIC (plasmid)
Construction: pCAT-Basic
Construct size (kb): 4.400000095367432
Features: marker(s): ampR
replicon: pMB1
reporter group: cat coding sequence
MCS: HindIII...XbaI
terminator: SV40 polyadenylation
promoter-cloning vector
Restriction digests of the clone give the following sizes (kb): ApaI--4.4; HindIII--4.4; XbaI--4.4.
Preparation of the vector for cloning includes linearization with ApaI, gel purification of the linearized vector, and treatment with T4 DNA polymerase in the presence of dATP.
The target sequence can be amplified using sequence specific primers modified at the 5' end to contain an additional 13 nt complementary to the vector.
The forward primer should contain 5'-CCTGCTCGTCTGA-3' followed by 12-15 nt target-specific sequence. The reverse primer should contain 5'-GGTGGTGCTCTGA-3' followed by 12-15 nt target-specific sequence.
Sequences amplified using these primers are also compatible with the pLUC/LIC vector (ATCC 87045).
Ligation-independent promoter-cloning vector, using chloramphenicol acetyltransferase as the reporter group.
The order of the major features in the plasmid is: ampR - pMB1 ori - HindIII - ApaI - XbaI - cat coding sequence - SV40 splice site and polyadenylation.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Haun RS, et al. Characterization of the human ADP-ribosylation factor 3 promoter: transcriptional regulation of a TATA-less promoter. J. Biol. Chem. 268: 8793-8800, 1993. PubMed: 8473323

Haun RS, et al. Rapid, reliable ligation-independent cloning of PCR products using modified plasmid vectors. BioTechniques 13: 515-518, 1992. PubMed: 1362067

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