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Size (kb): 9.3440008163452150
Vector: pCS19 (plasmid)
Promoters: Promoter lambda PR
Construction: YRp7-PHO5, URA3, CENXV, cI, pBR322
Construct size (kb): 9.344000816345215
Features: marker(s): ampR, URA3
promoter: lambda PR
replicon: pMB1, ARS1
repressor gene: cI
vector for constructing genomic libraries
vector permitting positive selection for inserts
Restriction digests of the clone give the following sizes (kb): PstI--6.8, 1.35, 1.2; BamHI--8.15, 1.2; EcoRI--4.9, 4.45.
Vector contains the following restriction sites (approximate kb from nt 1): BamHI--5.2, 6.4; BglII--0.6; EcoRI--0.0, 4.46; HindIII--0.84, 2.0, 4.21, 4.33; PstI--0.63, 1.8, 8.59; SalI--5.48; SmaI--0.9, 4.16; XbaI--2.43; XhoI--3.75.
Insertion into the BclI site inactivates the cI repressor, allowing expression of tetracycline resistance in recombinants.
Propagation in a dam- host is necessary for cleavage at the BclI site.
Shuttle vector for construction of genomic libraries that permits direct selection of recombinants.
The order of the major features in the plasmid is: EcoRI - ARS1 - SmaI - URA3 - XbaI - CEN15 - XhoI - BclI/cI - lambda PR - tetR - trp terminator - BamHI - pMB1 ori - ampR.
Sengstag C. The sequence of Saccharomyces cerevisiae cloning vector pCS19 allowing direct selection for DNA inserts. Gene 124: 141-142, 1993. PubMed: 8440476
Sengstag C, Hinnen A. The sequence of the Saccharomyces cerevisiae gene PHO2 codes for a regulatory protein with unusual amino acid composition. Nucleic Acids Res. 15: 233-246, 1987. PubMed: 3029672