Size (kb): 3.5069999694824220
Vector: pdeltablue (plasmid)
Promoters: Promoter lambda PL
Construct size (kb): 3.506999969482422
Features: insert detection: lacZ'
promoter: lambda PL
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BglI--1.75 (doublet); EcoRI--3.5; PstI--2.1, 1.45.
Restriction digests of the plasmid give the following sizes (kb): BamHI--3.507; BglI--1.784, 1.723; PstI--2.072, 1.435; PvuI--2.063, 1.444; PvuII--2.852, 0.655; SacI--3.257, 0.25.
Inserts can be cloned into the vector using a filled-in NcoI site and a MCS restriction site matching the 3' end of the insert. The vector supplies the AUG initiator codon.
The insert to be cloned should have a phosphorylated 5' blunt end with the first base corresponding to the first base of the first codon.
Expression vector producing proteins with the one additional N-terminal methionine. A modified lacZ' allows blue/white screening of colonies.
Constructed from pfXblue (ATCC 77333)
by deletion of lambda cII and coagulation factor Xa protease recognition sequences (nt 824-943).
The order of the major features of the plasmid is: pMB1 ori - ampR - lambdaPL promoter - NcoI/MCS/HindIII/modified lacZ'.
Brandt ME, Vickery LE. Expression and characterization of human mitochondrial ferredoxin reductase in Escherichia coli. Arch. Biochem. Biophys. 294: 735-740, 1992. PubMed: 1567230
Mark E Brandt, personal communication