pFL44S (ATCC® 77205)

Applications: YE-type (episomal) shuttle vectorshuttle vectorvector permitting visual detection of recombinants  /  Depositors: F Lacroute

Permits and Restrictions

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Designations pFL44S
Depositors F Lacroute
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.3189997673034670
Vector: pFL44S (plasmid)
Promoters: Promoter lac
Construction: pUC19
Construct size (kb): 4.318999767303467
Features: insert detection: lacZ'
marker(s): ampR, URA3
promoter: lac
replicon: pMB1, 2 micron
YE-type (episomal) shuttle vector
shuttle vector
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--4.4; BglII--3.3, 1.1; ClaI--3.9, 0.5.
The loss per generation (percentage having lost all the plasmids after 20 h of non-selective growth) is 13.4.
YE-type high copy number shuttle vector permitting visual detection of recombinants based on alpha complementation.
The order of the major features in this plasmid is: EcoRI/MCS/HindIII - URA3 - 2 micron ori - pMB1 ori - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470

Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589

Bach ML, et al. Evidence for transcriptional regulation of OMP-decarboxylase in yeast by hybridisation of mRNA to the yeast structural gene cloned in E. coli. Proc. Natl. Acad. Sci. USA 76: 386-390, 1979. PubMed: 370827

Chevallier MR, et al. Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeasts. Gene 11: 11-19, 1980. PubMed: 7002730

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
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