Size (kb): 3.5429999828338620
Vector: pFL35 (plasmid)
Promoters: Promoter lac
Construct size (kb): 3.542999982833862
Features: insert detection: lacZ'
marker(s): ampR, TRP1
YI-type (integrating) shuttle vector
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--3.7; BglII--2.9, 0.85; ClaI--3.7.
YI-type shuttle integration vector permitting visual detection of recombinants based on alpha complementation.
The order of the major features in this plasmid is: EcoRI/MCS/HindIII - TRP1 - pMB1 ori - ampR.
Broach JR, et al. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene 8: 121-133, 1979. PubMed: 395029
Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589