pMT70 (ATCC® 63013)

Organism: Mus musculus, mouse  /  Clone Type: Clone  /  Depositors: R Nusse

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Designations pMT70
Species Mus musculus, mouse
Depositors R Nusse
Construct size (kb): 5.400000095367432
Insert lengths(kb): 2.400000095367432
Gene product: wingless-type MMTV integration site 1, human homolog [Int1]
Insert Size (kb) 2.400
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Insert contains internal BamHI (1) and ClaI (1) sites.
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0, 2.4; EcoRI--5.4; HindIII--5.4; BamHI--5.4; consistent with deposited DNA.
A new nomenclature system has been proposed for Int-1 and related genes. It has been suggested that Int-1 become Wnt-1.
Contains cDNA of murine int-1 that encodes the complete protein. RNA can be synthesized in vitro from the SP6 vector promoter.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

van Ooyen A, Nusse R. Structure and nucleotide sequence of the putative mammary oncogene int-1; proviral insertions leave the protein-encoding domain intact. Cell 39: 233-240, 1984. PubMed: 6091914

Rijsewijk FA, et al. Construction of a retroviral cDNA version of the int-1 mammary oncogene and its expression in vitro. Nucleic Acids Res. 14: 693-702, 1986. PubMed: 3003692

Nusse R, et al. A new nomenclature for int-1 and related genes: the Wnt gene family. Cell 64: 231, 1991. PubMed: 1846319