Clostridium difficile (Hall and O'Toole) Prevot (ATCC® 43597)

Type Strain: no  /  Biosafety Level: 2

Permits and Restrictions

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Application
Enteric Research
Isolation
Feces, human
Belgium
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Preceptrol® no
Genotype Toxinotype: 0 RefRupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999
Type Strain no
Comments
Presence of tcdA and tcdB genes confirmed by PCR.
Binary toxin gene cdtB not amplified by PCR
Toxinotype 0
Ribotype 014
Medium ATCC® Medium 1053: Reinforced Clostridial medium (Oxoid CM149)
Growth Conditions
Max Temperature: 37.0°C
Min Temperature: 35.0°C
Atmosphere: Anaerobic
Genotype Toxinotype: 0 RefRupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999
Name of Depositor M Delmee
Isolation
Feces, human
Belgium
References

Delmee M, et al. Serogrouping of Clostridium difficile strains by slide agglutination. J. Clin. Microbiol. 21: 323-327, 1985. PubMed: 3980688

Delmee M, et al. Comparison of serogrouping and polyacrylamide gel electrophoresis for typing Clostridium difficile. J. Clin. Microbiol. 24: 991-994, 1986. PubMed: 3782463

feces of adult with diarrhea

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Cross References

Nucleotide (GenBank) : AJ132322 Clostridium difficile 16S rRNA gene (partial), internal transcriber spacer 1 (ITS1) and 23S rRNA (partial).

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
FAQs
  1. Toxin testing reference Clostridum dificile ATCC 43597

    The presence of these genes in ATCC® 43597 ™ was verified at the ATCC using PCR following the protocol described in the following reference:


    Date Updated: 1/11/2013