Yersinia rohdei Aleksic et al.

1. Open vial according to enclosed instructions. 
2. Using a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette.  Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube.  Mix well.
4. Use several drops of the suspension to inoculate a #260 agar slant and/or plate.
5. Incubate the tubes and plate at 26°C for 24 hours.

The following nucleotide sequences are available for this strain:

1. Nucleotide (GenBank) : AF366384 Yersinia rohdei 16S ribosomal RNA gene, partial sequence
2. Nucleotide (GenBank) : AX109664 Sequence 397 from Patent WO0123604

Additional information on this culture is available on the ATCC^® web site at www.atcc.org.

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