pSV2RbetaG [pSV2beta-globin] (ATCC® 37646)

Applications: expression vectorshuttle vector  /  Depositors: P Berg, M Dieckmann

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Designations pSV2RbetaG [pSV2beta-globin]
Depositors P Berg, M Dieckmann
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.7199997901916500
Vector: pSV2RbetaG (plasmid)
Promoters: Promoter SV40 early
Construct size (kb): 4.71999979019165
Features: marker(s): ampR
promoter: SV40 early
replicon: pMB1
terminator: SV40
enhancer: SV40 early
expression vector
shuttle vector
Restriction digests of the clone give the following sizes (kb): HindIII/BglII--4.23, 0.49; PvuII/PstI--2.22, l.55, 0.96; PstI--3.76, 0.96.
Expresses beta-globin in mammalian cells.
This plasmid includes the SV40 promoter (SV nt 270-5171), rabbit beta globin (487 bp), the SV40 splice site (SV nt4100-4710), the SV40 poly(A) (SV nt 1782-2770) and pBR322 (pBR nt 2067-4362).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Mulligan RC, Berg P. Expression of a bacterial gene in mammalian cells. Science 209: 1422-1427, 1980. PubMed: 6251549

Mulligan RC, et al. Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome. Nature 277: 108-114, 1979. PubMed: 215915

Paul Berg, personal communication

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