pSV2RbetaG [pSV2beta-globin] (ATCC® 37646)

Applications: expression vectorshuttle vector  /  Depositors: P Berg, M Dieckmann

Designations pSV2RbetaG [pSV2beta-globin]
Permits and Restrictions

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Depositors P Berg, M Dieckmann
Biosafety Level 1
Vector Information
Size (kb): 4.7199997901916500
Vector: pSV2RbetaG (plasmid)
Promoters: Promoter SV40 early
Construct size (kb): 4.71999979019165
Features: marker(s): ampR
promoter: SV40 early
replicon: pMB1
terminator: SV40
enhancer: SV40 early
expression vector
shuttle vector
Restriction digests of the clone give the following sizes (kb): HindIII/BglII--4.23, 0.49; PvuII/PstI--2.22, l.55, 0.96; PstI--3.76, 0.96.
Expresses beta-globin in mammalian cells.
This plasmid includes the SV40 promoter (SV nt 270-5171), rabbit beta globin (487 bp), the SV40 splice site (SV nt4100-4710), the SV40 poly(A) (SV nt 1782-2770) and pBR322 (pBR nt 2067-4362).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Mulligan RC, Berg P. Expression of a bacterial gene in mammalian cells. Science 209: 1422-1427, 1980. PubMed: 6251549

Mulligan RC, et al. Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome. Nature 277: 108-114, 1979. PubMed: 215915

Paul Berg, personal communication

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Shipping Information Distributed: freeze-dried