pBLCAT3 (ATCC® 37528)

Applications: vector for other usesvector permitting construction of fusion proteins  /  Depositors: B Luckow, G Schutz

Permits and Restrictions

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Designations pBLCAT3
Depositors B Luckow, G Schutz
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.3400001525878910
Vector: pBLCAT3 (plasmid)
Promoters: Promoter none
Construction: pUC18, SV40, cml
Construct size (kb): 4.340000152587891
Features: marker(s): ampR
promoter: none
replicon: pMB1
vector for other uses
vector permitting construction of fusion proteins
Restriction digests of the clone give the following sizes (kb): BamHI--4.4; HindIII--4.4; BglII--4.4; ClaI--uncut.
Developed to simplify construction of hybrid CAT genes.
The ClaI site appears to have been destroyed during cloning.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Luckow B, Schutz G. CAT constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elements. Nucleic Acids Res. 15: 5490, 1987. PubMed: 3037497

Shipped freeze-dried
Shipping Information Distributed: freeze-dried