pBLCAT2 (ATCC® 37527)

Applications: vector for other usesvector permitting construction of fusion proteins  /  Depositors: B Luckow, G Schutz

Permits and Restrictions

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Designations pBLCAT2
Depositors B Luckow, G Schutz
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.5000000000000000
Vector: pBLCAT2 (plasmid)
Promoters: Promoter HSV TK
Construction: pUC18, SV40, cml, herpes simplex virus TK promoter
Construct size (kb): 4.5
Features: marker(s): ampR
promoter: HSV TK
replicon: pMB1
vector for other uses
vector permitting construction of fusion proteins
Restriction digests of the clone give the following sizes (kb): BamHI--4.2; HindIII--4.2; BglII--4.2; ClaI--uncut; SmaI--4.3.
Plasmid to simplify construction of hybrid CAT coding sequences.
The ClaI site appears to have been destroyed during cloning.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Luckow B, Schutz G. CAT constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elements. Nucleic Acids Res. 15: 5490, 1987. PubMed: 3037497

Shipped freeze-dried
Shipping Information Distributed: freeze-dried