pUC118 (ATCC® 37462)

Applications: vector permitting production of single-stranded DNA  /  Depositors: P Dwivedi

Permits and Restrictions

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Designations pUC118
Depositors P Dwivedi
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.2000000476837160
Vector: pUC118 (phagemid)
Promoters: Promoter lac
Construction: M13, pUC18
Construct size (kb): 3.200000047683716
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1, M13
vector permitting production of single-stranded DNA
A pUC derivative containing the IG (intergenic) region of M13 for production of ssDNA.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Vieira J, Messing J. Production of single-stranded plasmid DNA. Methods Enzymol. 153: 3-11, 1987. PubMed: 3323803

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried stock of Escherichia coli containing the plasmid.