pUC118 (ATCC® 37462)

Applications: vector permitting production of single-stranded DNA  /  Depositors: P Dwivedi

Permits and Restrictions

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Designations pUC118
Depositors P Dwivedi
Biosafety Level 1
Vector Information
Size (kb): 3.2000000476837160
Vector: pUC118 (phagemid)
Promoters: Promoter lac
Construction: M13, pUC18
Marker(s):kanR
Construct size (kb): 3.200000047683716
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1, M13
Applications
vector permitting production of single-stranded DNA
Comments
A pUC derivative containing the IG (intergenic) region of M13 for production of ssDNA.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Vieira J, Messing J. Production of single-stranded plasmid DNA. Methods Enzymol. 153: 3-11, 1987. PubMed: 3323803

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried stock of Escherichia coli containing the plasmid.

Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map