pUC19 (ATCC® 37254)

Applications: expression vectorvector permitting construction of fusion proteins  /  Depositors: J Messing

Permits and Restrictions

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Designations pUC19
Depositors J Messing
Biosafety Level 1
Vector Information
Vector: pUC19 (phagemid)
Size (kb): 2.686
Construction: pUC71K
Marker(s): ampR (50 ug/ml)
Features: Insert detection: lacZ'; Promoter: lac; Replicon: pMB1; MCS: EcoRI...HindIII
Sequencing primers: M13(-21), M13 reverse
Applications
expression vector
vector permitting construction of fusion proteins
Comments
Expression vector encoding the beta-galactosidase alpha peptide (lacZ'), permitting blue-white visual detection of re combinants.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Vieira J, Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19: 259-268, 1982. PubMed: 6295879

Norrander J, et al. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene 26: 101-106, 1983. PubMed: 6323249

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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