pSV2-cat (ATCC® 37155)

Other IDs:

Nucleotide (GenBank) : VB0065 pSV2-Cat cloning vector.

 /  Applications: expression vectorshuttle vector  /  Depositors: BH Howard

Designations pSV2-cat
Permits and Restrictions

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Depositors BH Howard
Other IDs

Nucleotide (GenBank) : VB0065 pSV2-Cat cloning vector.

Biosafety Level 1
Vector Information
Size (kb): 5.1
Vector: pSV2-cat (plasmid)
Promoters: Promoter SV40
Construction: pSV2-betaG, Tn9
Construct size (kb): 5.1
Features: marker(s): ampR, cmlS
promoter: SV40
replicon: pMB1
expression vector
shuttle vector
Restriction digests of the vector give the following sizes (kb): HindIII--5.0; BamHI--5.0; BamHI/HindIII--3.5, 1.6; AccI--5.0.
Expresses chloramphenicol acetyltransferase in eukaryotic cell lines under the control of the simian virus 40 early promoter.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Gorman CM, et al. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol. Cell. Biol. 2: 1044-1051, 1982. PubMed: 6960240

Gorman CM, et al. The rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection. Proc. Natl. Acad. Sci. USA 79: 6777-6781, 1982. PubMed: 6294651

Gorman CHigh efficiency gene transfer into mammalian cellsIn: Gorman CDNA cloning2Washington, DCIRL Presspp. 143-190, 1985

Shipped freeze-dried