pSV2-cat (ATCC® 37155)

Other IDs:

Nucleotide (GenBank) : VB0065 pSV2-Cat cloning vector.

 /  Applications: expression vectorshuttle vector  /  Depositors: BH Howard

Permits and Restrictions

View Permits

Designations pSV2-cat
Depositors BH Howard
Other IDs

Nucleotide (GenBank) : VB0065 pSV2-Cat cloning vector.

Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.1
Vector: pSV2-cat (plasmid)
Promoters: Promoter SV40
Construction: pSV2-betaG, Tn9
Marker(s):ampR,cmlS
Construct size (kb): 5.1
Features: marker(s): ampR, cmlS
promoter: SV40
replicon: pMB1
Applications
expression vector
shuttle vector
Comments
Restriction digests of the vector give the following sizes (kb): HindIII--5.0; BamHI--5.0; BamHI/HindIII--3.5, 1.6; AccI--5.0.
Expresses chloramphenicol acetyltransferase in eukaryotic cell lines under the control of the simian virus 40 early promoter.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Gorman CM, et al. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol. Cell. Biol. 2: 1044-1051, 1982. PubMed: 6960240

Gorman CM, et al. The rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection. Proc. Natl. Acad. Sci. USA 79: 6777-6781, 1982. PubMed: 6294651

Gorman CHigh efficiency gene transfer into mammalian cellsIn: Gorman CDNA cloning2Washington, DCIRL Presspp. 143-190, 1985

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map