pSV2-neo (ATCC® 37149)

Applications: expression vector shuttle vector  /  Depositors: P Berg

Designations pSV2-neo
Depositors P Berg
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.729
Vector: pSV2-neo (plasmid)
Promoters: Promoter SV40 early
Construction: SV40, Tn5, pBR322
Construct size (kb): 5.729
Features: marker(s): G418R
marker(s): ampR
promoter: SV40 early
replicon: SV40
replicon: pMB1
restriction site: HindIII
expression vector
shuttle vector
Restriction digests of the clone give the following sizes (kb): PvuII--4.2, 0.74, 0.61, 0.36; BamHI--5.8; EcoRI--5.8; PstI--2.45, 1.40, 0.94 (doublet).
Provides dominant selectable marker for resistance to antibiotic G418 in mammalian cell lines.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Southern PJ, Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Appl. Genet. 1: 327-341, 1982. PubMed: 6286831

Shipping Information

Shipped:  Frozen glycerol stock of  E. coli containing the plasmid