pSV2-neo plasmid in Escherichia coli (ATCC® 37149)

Applications: Expression vector Shuttle vector  /  Depositors: P Berg, Stanford University Medical Center

Designations pSV2-neo plasmid in Escherichia coli
Permits and Restrictions

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Depositors P Berg, Stanford University Medical Center
Biosafety Level 1
Host Escherichia coli HB101
Vector Information
Size (kb): 5.729
Vector: pSV2-neo (plasmid)
Promoters: Promoter SV40 early
Construction: SV40, Tn5, pBR322
Marker(s): G418R,ampR
Construct size (kb): 5.729
Features: marker(s): G418R
marker(s): ampR
promoter: SV40 early
replicon: SV40
replicon: pMB1
restriction site: HindIII
Expression vector
Shuttle vector
Restriction digests of the clone give the following sizes (kb): PvuII--4.2, 0.74, 0.61, 0.36; BamHI--5.8; EcoRI--5.8; PstI--2.45, 1.40, 0.94 (doublet).
Provides dominant selectable marker for resistance to antibiotic G418 in mammalian cell lines.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C

Southern PJ, Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Appl. Genet. 1: 327-341, 1982. PubMed: 6286831

Shipped frozen
Shipping Information Glycerol stock of  E. coli containing the plasmid