YEp13 (ATCC® 37115)

Applications: YE-type (episomal) shuttle vectorshuttle vectorvector for constructing genomic libraries  /  Depositors: JR Broach

Permits and Restrictions

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Designations YEp13
Depositors JR Broach
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 10.6669998168945300
Vector: YEp13 (plasmid)
Promoters: Promoter none
Construction: pBR322, Saccharomyces cerevisiae LEU2, 2 micron ori
Construct size (kb): 10.66699981689453
Features: marker(s): LEU2
marker(s): ampR
marker(s): tetR
promoter: none
replicon: 2 micron
replicon: pMB1
terminator: none
enhancer: none
YE-type (episomal) shuttle vector
shuttle vector
vector for constructing genomic libraries
Restriction digests analyzed on agarose gels give the following sizes (kb): PstI--5.5, 4.0, 0.9; HindIII--10.7.
Transforms leu2- yeast to Leu+ at 10E3 to 10E4 colonies per microgram of DNA.
YEp13 is mitotically stable in cir+ strains.
A YE-type S. cerevisiae/E. coli episomal shuttle vector.
An EcoRI fragment containing the 2 micron origin was cloned into the EcoRI site of pBR322. The resulting plasmid was partially digested with PstI, and a PstI fragment containing yeast LEU2 was cloned into the 2 micron PstI site.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Broach JR, et al. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene 8: 121-133, 1979. PubMed: 395029

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component of:ATCC 87635
Shipped frozen
Shipping Information

Shipped:  Frozen glycerol stock of Escherichia coli containing the plasmid.