YIp5 [pRB12] (ATCC® 37061)

Applications: YI-type (integrating) shuttle vectorintegrating vectorshuttle vector  /  Depositors: D Botstein

Permits and Restrictions

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Designations YIp5 [pRB12]
Depositors D Botstein
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.5409998893737790
Vector: YIp5 (plasmid)
Construction: pBR322, lambda590-Sc2904
Marker(s):URA3,ampR,tetR
Construct size (kb): 5.540999889373779
Features: marker(s): ampR, tetR, URA3
replicon: pMB1
terminator: none
enhancer: none
Applications
YI-type (integrating) shuttle vector
integrating vector
shuttle vector
Comments
YI-type integrating shuttle vector.
This was constructed by cloning the 1.1 kb HindIII fragment containing URA3 from lambda590-Sc2904 into the AvaI site of pBR322. The 5' - 3' orientation of URA3 is clockwise; SmaI and AvaI sites in the 3' of URA3 are close to the PvuII site of pBR322.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Related Products
component of:ATCC 77188
Shipped frozen
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
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