YRp7 [pRB16] (ATCC® 37060)

Applications: YR-type (replicating) shuttle vectorshuttle vector  /  Depositors: D Botstein

Permits and Restrictions

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Designations YRp7 [pRB16]
Depositors D Botstein
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.817
Vector: YRp7 (plasmid)
Promoters: none
Construction: pBR322, lambdagt-Sc4101
Construct size (kb): 5.817
Features: marker(s): TRP1
marker(s): ampR
marker(s): tetR
promoter: none
replicon: ARS1
replicon: ROP
replicon: pMB1
terminator: none
enhancer: none
YR-type (replicating) shuttle vector
shuttle vector
A general purpose replicating Saccharomyces cerevisiae/Escherichia coli shuttle vector.
YRp7 was constructed by inserting a 1.4 kb EcoRI fragment containing TRP1 and ARS1 from lambdagt-Sc4101 into pBR322. The ARS1 fragment is in a 0.85 kb EcoRI-HindIII subfragment.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried E. coli containing the plasmid

Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map