pC194 (ATCC® 37034)

Other IDs:

Nucleotide (GenBank) : VB0132 pC194 cloning vector for Bacillus subtillis.

 /  Applications: shuttle vector  /  Depositors: D Dubnau

Permits and Restrictions

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Designations pC194
Depositors D Dubnau
Other IDs

Nucleotide (GenBank) : VB0132 pC194 cloning vector for Bacillus subtillis.

Biosafety Level 1
Vector Information
Size (kb): 2.9000000953674320
Vector: pC194 (plasmid)
Construction: natural isolate
Marker(s):cmlR
Construct size (kb): 2.900000095367432
Features: marker(s): cmlR
replicon: pC194
Applications
shuttle vector
Comments
natural isolate
General purpose plasmid vector for cloning in Bacillus.
Media ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37.0°C
References

Gryczan TJ, et al. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J. Bacteriol. 134: 318-329, 1978. PubMed: 418061

Ehrlich SD. DNA cloning in Bacillus subtilis. Proc. Natl. Acad. Sci. USA 75: 1433-1436, 1978. PubMed: 418413

Horinouchi S, Weisblum B. Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance. J. Bacteriol. 150: 815-825, 1982. PubMed: 6950931

Shipped frozen
Shipping Information

Shipped:  Frozen glycerol stock of B. subtilis containing the plasmid.

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 37034