pACYC184 (ATCC® 37033)

Depositors: SN Cohen

Permits and Restrictions

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Designations pACYC184
Depositors SN Cohen
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host Escherichia coli PM191
Vector Information Vector size (kb): 4.244
Markers: cmlR, tetR
DNA Seq. Acc.: X06403

Type of vector: plasmid
Cloning sites: EcoRI HindIII BamHI SalI
Construction: p15A, pSC101, Tn9
Host range: Escherichia coli
Features):
marker(s): cmlR, tetR
replicon: p15A

Suggested host(s):
Escherichia coli chi-1776 (ATCC® 31244™)
Escherichia coli chi-1776 (ATCC® 31537™)
Escherichia coli chi-1776 (ATCC® 39234™)
Escherichia coli chi-1776 (ATCC® 53705™)
Escherichia coli C600 (ATCC® 23724™)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; EcoRI/BamHI--2.4, 1.9.
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; EcoRI/BamHI--2.4, 1.9.
Low copy number plasmid vector containing the replication system of miniplasmid p15A.
Media ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37°C
References

Chang AC, Cohen SN. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134: 1141-1156, 1978. PubMed: 149110

Rose RE. The nucleotide sequence of pACYC184. Nucleic Acids Res. 16: 355, 1988. PubMed: 3340533

Shipped frozen
Shipping Information Frozen glycerol stock of Escherichia coli containing the plasmid.
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map