pACYC177 (ATCC® 37031)

Other IDs:

Nucleotide (GenBank) : VB0109 Cloning vector pACYC177.

 /  Depositors: SN Cohen

Permits and Restrictions

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Designations pACYC177
Other IDs

Nucleotide (GenBank) : VB0109 Cloning vector pACYC177.

Depositors SN Cohen
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.9400000572204590
Vector: pACYC177 (plasmid)
Construction: p15A, Tn903, Tn3
Construct size (kb): 3.940000057220459
Features: marker(s): ampR, kanR
replicon: p15A
Low copy number plasmid vector containing the replication system of miniplasmid p15A.
When HincII is nucleotide 1, Tn3 region extends from 3026-756 and beta-lactamase from 369-615. The promoter and part of the coding region for the Tn3 repressor (3025-3515) have been deleted. The Tn903 (1595-3025) region includes kanR (1922-2734).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Chang AC, Cohen SN. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134: 1141-1156, 1978. PubMed: 149110

Rose RE. The nucleotide sequence of pACYC177. Nucleic Acids Res. 16: 356, 1988. PubMed: 3340534

Shipped frozen
Shipping Information

Shipped:  Frozen glycerol stock of  E. col containing the plasmid.