pUB110 (ATCC® 37015)

Applications: shuttle vector  /  Depositors: D Dubnau

Permits and Restrictions

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Designations pUB110
Depositors D Dubnau
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host Bacillus subtilis BD170
Vector Information
Size (kb): 4.548
Vector: pUB110 (plasmid)
Cloning Sites: EcoRI XbaI BglII BamHI PvuII TacI
Promoters: Promoter none
Construction: natural isolate
Host Range: Bacillus sp., Staphylococcus aureus
Marker(s): kanR,neoR
Construct size (kb): 4.548
Features: marker(s): kanR, neoR
promoter: none
replicon: pUB110
shuttle vector
natural isolate
general purpose plasmid vector for cloning in Bacillus
Media ATCC® Medium 1179: VY medium
Growth Conditions
Temperature: 37°C

Gryczan TJ, et al. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J. Bacteriol. 134: 318-329, 1978. PubMed: 418061

Gryczan T, et al. Characterization of chimeric plasmid cloning vehicles in Bacillus subtilis. J. Bacteriol. 141: 246-253, 1980. PubMed: 6243622

McKenzie T, et al. The nucleotide sequence of pUB110: some salient features in relation to replication and its regulation. Plasmid 15: 93-103, 1986. PubMed: 3010356

McKenzie T, et al. A revision of the nucleotide sequence and functional map of pUB110. Plasmid 17: 83-84, 1987. PubMed: 3033723

Shipped frozen