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Escherichia coli (Migula) Castellani and Chalmers (ATCC® 35150)

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Strain Designations: EDL 931  /  Type Strain: no  /  Biosafety Level: 2
[Associated with hemorrhagic colitis,

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Verified By Whole-genome Sequencing
Classification Enterobacteriaceae, Escherichia
Strain Designations EDL 931
Feces, human
Biosafety Level 2
[Associated with hemorrhagic colitis, ref]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Antigenic Properties Serotype O157:H7
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype Multilocus Sequence Type (MLST): ST-32
Genome Sequenced Strain


Type Strain no
Antigenic Properties Serotype O157:H7
Presence of eaeA, stx1, and stx2 genes confirmed by PCR.
Cytotoxic activity
Hemorrhagic colitis
Growth on Novobiocin (10 mg/L)
Growth on Tellurite (0.8 mg/L)
Medium ATCC® Medium 18: Trypticase Soy Agar/Broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Genotype Multilocus Sequence Type (MLST): ST-32
Name of Depositor GK Morris
Chain of Custody
ATCC <-- GK Morris <-- JG Wells
Feces, human

Beery JT, et al. Cytotoxic activity of Escherichia coli O157:H7 culture filtrate on the mouse colon and kidney. Curr. Microbiol. 11: 335-342, 1984.

Riley LW, et al. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N. Engl. J. Med. 308: 681-685, 1983. PubMed: 6338386

Cross References

Nucleotide (GenBank) : AF305917 Escherichia coli O157:H7 nonfunctional beta-glucuronidase (uidA) gene, complete cds.

Nucleotide (GenBank) : AWXM00000000 Escherichia coli ATCC 35150, whole genome shotgun sequencing project

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • This item is listed on the Commerce Control List of the U.S. Department of Commerce and is distributed only within the 50 United States. Distribution requires completion of a Customer Acceptance of Responsibility (CAR) for Commerce Control List (CCL) Biologicals form.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
  1. Accessing genome sequencing data

    Date Updated: 9/30/2019

  2. Criteria for reference quality genomes

    Date Updated: 9/30/2019