Dulbecco's Modified Eagle's Medium (DMEM)

30-2002 ^™

ATCC®-formulated Dulbecco’s Modified Eagle’s Medium (DMEM) is a high-quality, sterile liquid medium designed for culturing a broad range of mammalian cells, such as ATCC’s 293T (ATCC CRL-3216™). Based on the classic DMEM formulation, it contains elevated glucose, amino acids, vitamins, and inorganic salts to support robust cell growth and metabolism. The medium includes sodium bicarbonate for buffering and phenol red for pH indication. Suitable for applications in cancer research, bioproduction, and cell physiology. Supplement with serum as needed.

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Product category

Media

Formulation

Contains 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. NOTE: This reduced level of sodium bicarbonate (NaHCO₃, 1.5 g/L) is intended for use in a 5% CO₂ in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO₂.

Comments

Dulbecco's Modified Eagle's Medium (DMEM) is modified to contain 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate.

This reduced level of sodium bicarbonate (NaHCO₃, 1.5 g/L) is intended for use in 5% CO₂ in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO₂.

Applications

Cell culture

Cell growth and viability

Volume

500 mL

Storage conditions

2°C to 8°C

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Documentation

Product sheet

Certificate of origin

Media formulation

ATCC determined that a biosafety level is not applicable to this material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to complete your own risk assessment and understand any potential hazards associated with the material per your organization’s policies and procedures and any other applicable regulations as enforced by your local or national agencies.

General

Specific applications: Dulbecco's Modified Eagle's Medium (DMEM) modified to contain 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate.

Handling information

Handling notes: Store medium at 2°C to 8°C in the dark when not in use.

Quality control specifications

Bacterial and fungal testing: Not detected
Mycoplasma contamination: Not detected
Endotoxin: ≤ 0.5 EU/mL
Osmolality: 270 to 366 mOsm/kg
pH: 7.0 to 7.4
Growth promotion: Pass

History

Depositors: ATCC

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

References

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