Acetobacter estunensis (Carr) Lisdiyanti et al.

1. Open vial according to enclosed instructions.  
2. Using a single tube of #1 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette.  Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube.  Mix well.
4. Use several drops of the suspension to inoculate a #1 agar slant and/or plate.
5. Incubate the tubes and plate at 26^oC for 48-72 hours.

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