The Art and Science of Growing Animal Viruses in the Laboratory

Best Practices in Virus Culture

10/12/2017


Time: 12:00 PM Eastern Standard Time

Abstract:

Employing good aseptic technique and understanding the specific culture requirements of a strain are essential for optimal viral growth. In this webinar, we will discuss the basic principles for propagating animal viruses in the laboratory. Here, we will highlight best practices for making good virus stocks with an emphasis on the art and science of virus culture that should be followed to help avoid contamination and cross-contamination. Further, we will provide an in-depth look at viral culturing practices in both cell culture and embryonated chicken eggs using Influenza virus as an example.

Key Points:

  • Good aseptic technique alone is not sufficient to grow and maintain virus stocks
  • Host cell culture authentication is important for growing a good stock of virus
  • The nature of biomaterials used in culturing viruses in cells and the tendency for viruses to mutate are the primary challenges in obtaining consistent end results

Presenters

Dr. Sujatha R. Rashid

Sujatha R. Rashid, Ph.D.,
Scientist, ATCC

Dr. Sujatha R. Rashid is a Virology Scientist at ATCC. She received her Ph.D. from the Christian Medical College, Vellore, India, where she also received training in medical and clinical virology. She has lead management oversight of the virology collection in Microbiology and Infectious Diseases Biological Resources Repository (MID-BRR), also known publicly as BEI Resources, which is a government-funded biorepository managed by ATCC.
Jessica M. Shifflett

Jessica M. Shifflett,
Scientist, ATCC

Jessica M. Shifflett has 10 years of virology and assay development experience at ATCC. She earned her M.S. in Microbiology at George Mason University working with Dr. Yuntao Wu.