L-M(TK-) cells (ATCC CCL-1.3)
were transfected with a Wnt-3A expression vector and stable clones were selected in medium containing G418.
The Wnt-3A gene encodes a secreted glyoprotein with a variety of signaling effects. Wnt genes control many of the patterning and growth events during embryonic development.
The cells secrete biologically active Wnt-3A protein. They are presently the best source for production of Wnt-3A conditioned medium.
Since the conditioned medium contains other factors besides the Wnt-3A protein, it is necessary to control any experiments involving the Wnt-3A conditioned medium with control conditioned medium from the parental cell line (ATCC CRL-2648)