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Grainy, pink and yellow spheres of Middle East respiratory syndrome coronavirus.

Viral Metagenomics and the Use of Standards: From Biology to Clinical Applications

June 25, 2020, at 12:00 PM ET

Abstract

Viruses can infect bacteria as well as a myriad of eukaryotic cells. Virus membership and function within the human microbiome are being revealed with the advent of metagenomics, and knowledge on these communities will continue to expand with the application of new sequencing technologies and analysis tools. This information is readily translating into clinical applications, which include, but are not limited to, the rapid sequence identification of pathogenic viruses and phage therapy. However, without appropriate standards and controls, researchers are unable to make meaningful cross-study comparisons or assess biases that can be introduced at different stages of a viral metagenomics pipeline. As the field of viral metagenomics translates into clinical and other applications, the implementation of viral standards and controls is of increasing importance. 

Key Points

  • Implementation of viral standards and controls can help determine biases that could be potentially introduced at different stages of a viral metagenomics pipeline.
  • Identification of biases in a viral metagenomics study provides the opportunity to troubleshoot and benchmark reagents and analysis tools. 
  • Viral standards may evolve to mimic simple and more complex viral communities in diverse environments.

Watch The Presentation

Presenter

Tasha M. Santiago-Rodriguez, headshot.

Tasha M. Santiago-Rodriguez, PhD

Data Analyst/Project Manager (Instructor), The Alkek Center for Metagenomics and Microbiome Research, Baylor College of Medicine

Dr. Tasha M. Santiago-Rodriguez is a Data Analyst/Project Manager at the Alkek Center for Metagenomics and Microbiome Research at the Baylor College of Medicine. Previously, she worked as a Senior Data Scientist at Diversigen in Houston, Texas a microbiome service company where she was part of the R&D team developing new methods to better interpret microbiome studies. She received a PhD from the University of Puerto Rico in Public Health Water Microbiology, characterizing bacteriophages as indicators of human fecal contamination. Dr. Santiago-Rodriguez conducted postdoctoral research at the University of California, San Diego, focusing on virome research related to Methicillin-Resistant Staphylococcus aureus (MRSA) infections, urinary tract infections, and antibiotic use. She was also a recipient of a postdoctoral fellowship from the Howard Hughes Medical Institute to conduct research on ancient microbiomes and viromes at the California Polytechnic State University, San Luis Obispo. She later joined the American Type Culture Collection (ATCC) Center for Translational Microbiology to develop standards and controls for microbiome research. Dr. Santiago-Rodriguez has published over 50 articles and book chapters in water microbiology, bacteriophages, as well as ancient and modern microbiomes and viromes.