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Red, purple, green, and yellow A549 CRISPR cells.

Best Practices for Cell Culture

May 21, 2020, at 12:00 PM ET


Over its 95 years of supplying biomaterials to the research community, ATCC has been recognized as the gold standard for cryopreserved cells, culture reagents, and culture resources. Through rational experimentation, we have identified the best practices for cell culture; the sum of these tips and techniques has developed into a knowledge base that is second to none. In this webinar, we will tap into that vast experience and share the best practices for culturing cells that ensure optimal results and performance. The information delivered in this webinar will cover all aspects of successful cell culture, including culture initiation, expansion, authentication, and cryopreservation.

Key Points

  • There are many cell models; choosing the correct one for your workflow will add efficiency and productivity to your work.
  • When culturing specialty cells, such as stem cells or primary cells, certain considerations regarding the choice of media and reagents must be taken.
  • Using misidentified or cross-contaminated cell lines in experiments can invalidate experimental results; therefore, authenticating cell lines should be part of your cell culture workflow.
  • Mycoplasma infection can chronically affect the well-being of cells in culture without being detected by visual observation; multiple screening methods can be used to identify contamination.
  • Tools such as proliferation assays and cryo-containers can aid the maintenance of cell health.

Watch The Presentation


Steve Budd, headshot.

Steven Budd, MS, MBA

Product Specialist, ATCC

Steven Budd is a Product Specialist that manages the cell culture reagents at ATCC. He has 6 years of experience in the product management of scientific tools. Before that, he gained 4 years of experience in biomedical research and cell culture as a research specialist at the University of North Carolina at Chapel Hill. Mr. Budd has a M.S. in Biology from the University of North Carolina at Wilmington and an M.B.A. from North Carolina State University.