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Purple and red HTB-9 B-Catenin monolayer cells.

Cell Culture Fundamentals: Your Questions Answered

Aug 01, 2019 at 12:00 PM ET

Abstract

The successful cultivation of cells is critical for performing cell-based assays, yet even the most experienced scientists can encounter issues with their cultures. In this webinar, we will provide some best practices for cell culture, including tips and techniques for initiation, expansion, authentication, and cryopreservation. After the presentation, we invite you to join in on the discussion as a panel of ATCC cell culture experts answer your questions.

Key Points

  • Using misidentified or cross-contaminated cell lines in experiments can invalidate research efforts; therefore, authenticating cell lines should be part of your cell culture work flow.
  • When culturing specialty cells, such as stem cells or primary cells, certain considerations regarding the choice of media and reagents must be taken.
  • Cryopreserving cells under optimal conditions is critical for maintaining cell viability. 

Watch The Presentation

Presenters

Kevin Grady, headshot.

Kevin Grady, BS

Senior Product Line Business Manager, ATCC

Kevin Grady is the Senior Product Line Business Manager for Cell Biology at ATCC. He has been with ATCC for 8 years; prior to ATCC, he held positions at Lonza as Worldwide Product Manager and Director of Scientific Support. Kevin has a long history in the life science industry additionally serving as Director of Scientific Support at Amaxa and Manager of Technical Support at Life Technologies. Mr. Grady has always found great satisfaction in helping researchers learn how to use available products and tools to be more productive and successful in reaching their research goals.

Steve Budd, headshot.

Steven Budd, MS, MBA

Product Line Business Specialist, ATCC

Steven Budd is a Product Line Business Specialist that manages the cell culture reagents at ATCC. He has 4 years of experience in the product management of scientific tools. Before that, he gained 4 years of experience in biomedical research and cell culture as a research specialist at the University of North Carolina at Chapel Hill. Mr. Budd has a M.S. in Biology from the University of North Carolina at Wilmington and an M.B.A. from North Carolina State University.