ATCC Microbiology

Authenticated reference materials are essential for assay reproducibility and data integrity. To ensure the quality of these cultures, it is imperative that they are well-characterized and carefully managed through preservation and storage protocols that maintain the genotype and phenotype. In this webinar, we will discuss the best practices for stock maintenance with regard to passage, storage, recovery, and microbial authentication, and how ATCC manages these through the seed stock concept, adhering to the specific needs of each culture, and polyphasic strain authentication.

Key Points

• Minimizing passage number reduces the likelihood of contamination, genetic drift, mutation, and phenotypic variation
• Proper storage and recovery of frozen and freeze-dried cultures protects post-preservation viability
• Combining phenotypic, genotypic, proteotypic, and functional analyses provides the best possible strain authentication and characterization