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Seeing is Believing: Reporter-Labeled Microbial Control Strains

May 21, 2015 at 12:00 PM ET

Abstract

Experimental controls are essential for establishing that protocols, methods, and instruments are functioning correctly and efficiently. ATCC, a world leader in high-quality biological controls, now offers reporter-labeled microbial control strains, including Escherichia coli, Shiga toxin-producing E. coli (STEC), Pseudomonas aeruginosa, Salmonella enterica, and Shigella flexneri. These labels allow for easy, visual discrimination between control strains and sample strains to minimize errors caused by cross-contamination in the laboratory. In this webinar, we will discuss the development and evaluation of reporter-labeled controls, and will examine their applications in microbial detection and quantification, microscopy, host-pathogen interaction studies, and food testing.

Presenters

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Jodie Lee, MS

Lead Biologist, ATCC

Questions and Answers

Are there licensing requirements to consider when choosing between GFP- and NanoLuc-labeled strains?

GFP products are distributed under the terms of the ATCC MTA and a Limited Use License from Life Technologies. The commercial use of GFP and research use of GFP by for-profit institutions both require a license from Life Technologies. Many large companies already have a license in place. Academic and government institutions are generally exempt from from this requirement. The Limited Use License is available on the product pages of our website. NanoLuc and Nano-Glo products are distributed under the terms of the ATCC MTA and a Limited Use License from Promega. Promega does not require for-profit institutions and academic and government institutions to have a license for the research use of NanoLuc and Nano-Glo. The commercial use of NanoLuc and Nano-Glo requires a license from Promega. The Limited Use License is available on the product pages of our website.

How long is the NanoLuc label visible?

The reporter will produce an intense blue glow-response signal until the substrate runs out or the reaction dries. The light is generally visible for several minutes.

What are the storage conditions for the NanoLuc substrate?

The labeled strains will be shipped to you on dry ice with the substrate and lysis buffer. Store the lysis buffer and the substrate separately at -20°C. The strains should be stored at -70°C or colder. Make the reaction solution by mixing the substrate and the lysis buffer. The reaction solution will keep for up to a week at 4°C, but will slowly lose activity. For the brightest response, prepare only as much fresh reaction solution as required for each experiment, storing the substrate and lysis buffer separately until needed.