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Nitratireductor zodletonensis

TSD-294

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Nitratireductor zodletonensis strain ZSWI3 is the type strain of a novel bacterial species isolated from sediment in the Zodletone Spring, located on Zodletone Mountain in Kiowa County, Oklahoma, USA.
Product category
Bacteria
Classification
Phyllobacteriaceae
Strain designation
ZSWI3
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Sulfur-rich sediment, Zodletone Spring
Geographical isolation
United States; Oklahoma
Product format
Frozen
Storage conditions
-80°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Preceptrol
No

Characteristics

Susceptibility profile
Susceptible
Carbenicillin
Cefaclor
Cefoxitin
Ceftriaxone
Chloramphenicol
Ciprofloxacin
Doxycycline
Tetracycline
Erythromycin
Nalidixic Acid
Oxytetracycline
Sulfathiazole
Comments
Depositor-provided phenotypic description: Cells are gram-negative, motile rods, approximately 2.2 x 0.6 μm. Cells are positive for oxidase and catalase. Colonies are opaque pale yellow to beige in color, circular, slightly raised and mucoid with a neat margin 2-3 mm in diameter after growth on TSA for 3 days. Optimal growth occurs at 30-35°C, between pH 7-8, and in the presence of 0.5-2% (w/v) NaCl. Reduces nitrate to nitrite. Facultatively anaerobic and utilizes a wide variety of substrates as sole carbon sources. Sensitive to (μg per disc): tetracycline (30), doxycycline (30), oxytetracycline (30), carbenicillin (100), cefoxitin (30), ceftriaxone (30), cefaclor (30), ciprofloxacin (5), nalidixic acid (30), chloramphenicol (30), sulfathiazole (250), and erythromycin (15). The dominant fatty acids are C18:1ω7c and C19:0ω7c cyclo.
Type strain description
This culture provided to the ATCC type strain depository is neither produced nor characterized by ATCC. No technical information is available on this material. Refer to depositor for technical information on this strain.
Technical information
ATCC Product Experience does not have technical information on type strain deposits that are not fully characterized. Additional information can be found in the depositor’s publication.

Handling information

Temperature
30°C
Atmosphere
Aerobic
Depositor-recommended growth conditions
It is recommended that this strain be grown aerobically in tryptic soy broth (TSB) or on tryptic soy agar (TSA), at 30°C with media prepared as recommended by the manufacturer.
Handling procedure
1 day of incubation in broth media is needed to obtain log phase growth. Isolated colonies on present on agar/solid media after 3 days. Subculturing is performed every 7-10 days onto fresh TSB or TSA media. 

Quality control specifications

Verification method
Whole-genome Sequencing

History

Depositors
L Krumholz, University of Oklahoma
Type of isolate
Environmental
Year of origin
2019

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Disclosures

ATCC is a private, nonprofit biological resource center (BRC) and research organization that holds deposits of new type strains. ATCC tests for viability and identity upon the initial deposit of type strains. For those strains with a “TSD” designation, no further testing is performed and these strains are made available on behalf of the depositor per the requirements of the International Journal of Systematic and Evolutionary Microbiology (IJSEM), published by the Society for General Microbiology (SGM), and the International Committee on Systematics of Prokaryotes (ICSP).

ATCC may fully accessions new type strains into its general culture collection. At that time, ATCC will provide an “ATCC” designation to the strain, fully characterize the strain, and provide a Certificate of Analysis with authentication data for that specific item.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

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