Pythiogeton zeae Jee et al.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
1. To thaw a frozen ampule, place in a 37 °C water bath, until just thawed (approximately 2.5 minutes for a plastic vial). Immerse the ampule just enough to cover the frozen material. Do not agitate the ampule.
2. Immediately after thawing, aseptically transfer the contents of the vial onto appropriate moist solid medium and a liquid medium. This strain may revive more readily in liquid media such as ATCC 200: YM broth (= Difco 0711 = BD 271120) or ATCC 5103: nutrient broth +0.1% yeast extract. Added thiamine may encourage growth.
3. Incubate cultures at recommended temperature.
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Jee HJ, et al. Pythiogeton zeae sp. nov. causing root and basal stalk rot of corn in Korea. Mycologia 92: 522-527, 2000.