Pythiogeton zeae Jee et al.
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1. To thaw a frozen ampule, place in a 37 °C water bath, until just thawed (approximately 2.5 minutes for a plastic vial). Immerse the ampule just enough to cover the frozen material. Do not agitate the ampule.
2. Immediately after thawing, aseptically transfer the contents of the vial onto appropriate moist solid medium and a liquid medium. This strain may revive more readily in liquid media such as ATCC 200: YM broth (= Difco 0711 = BD 271120) or ATCC 5103: nutrient broth +0.1% yeast extract. Added thiamine may encourage growth.
3. Incubate cultures at recommended temperature.
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Jee HJ, et al. Pythiogeton zeae sp. nov. causing root and basal stalk rot of corn in Korea. Mycologia 92: 522-527, 2000.