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Paracoccidioides lutzii Teixeira et al.

MYA-826

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An ampoule containing viable cells (yeast cells, spores, or agar cubes with mycelia) suspended in cryoprotectant.
Product category
Fungi
Classification
Fungi, Dikarya, Ascomycota, Pezizomycotina, Eurotiomycetes, Eurotiomycetidae, Onygenales, Ajellomycetaceae, Paracoccidioides
Strain designation
Pb01 [01]
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Clinical isolate
Geographical isolation
Brazil; Goias; Goiania
Product format
Frozen
Storage conditions
-80°C or colder
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Documentation

Biosafety Icon Biosafety Level 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
RAPD analysis
Biomedical Research and Development Material
Preceptrol
No

Characteristics

Comments
Highly virulent
Differential expression of proteins PbY20 (by yeast form) and PbM46 (by mycelial form)
Protein synthesis patterns
Effect of amphotericin B
Expression of gp43
Differential expression of gene for heat shock protein, Hsp70
RAPD analysis
Genome sequencing strain (University of Brasil, Brasil).
This fungus was previously known as Paracoccidioides brasiliensis
Growth is restricted and heaped on all media and is most robust on Rabbit Blood agar. This strain does not grow well in broth.

Handling information

Medium
Temperature
35-37°C
Atmosphere
Aerobic
Handling procedure
Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
  1. To thaw a frozen ampoule, place in a 55°C water bath, until just thawed (approximately 90 seconds).  Immerse the ampoule just sufficient to cover the frozen material.  Do not agitate the ampoule.
  2. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
  3. Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-4 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Handling notes
Growth is restricted and heaped on all media and is most robust on Rabbit Blood agar. This strain does not grow well in broth.
Additional information on this culture is available on the ATCC web site at www.atcc.org.

Quality control specifications

Sequenced data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28/26S ribosomal RNA gene, partial sequence

AAGGATCATTAACGCGCCGTGGGGGGACGGGGCCCGATCGGGTTCCCCGACCCTCTCACCTGGCCACCCTTGTCTATCCTACCTGTTGCTTCGGCGGGCCTGCAGCGATGCTGCCGGGGGGGCTTGGCCTCCCGGGCTCGTGCCCGCCGGGGACACCGTTGAACTTCTGGTTCGGAGCTTTGACGTCTGAGACCCATCATAATCAGTGAAAACTTTCAACAACGGATCTCTTGGTTCCGACATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCAAGCGCGGCTTGTGTGTTGGGCCCGCGTCCCCCCATGGACGTGCCCGAAAAGCAGCGGCGGCGTCGCGTTCCGGTGCCCGAGCGTATGGGGCTTCGTCACACGCTCTCAGAGGCCCGGCCGGCCCCGGCCCCCACTCATCGACCCCGGCGGGGGGGAAAGTAAGTCTTCTCTCGACATCTTCCCCTCTTCCGACCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAG


D1D2 region of the 28/26S ribosomal RNA gene

CATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAGAGCTCAAATTTGAAATCTGGCTCCTTTGGGGCCCGAGTTGTAATTTGTAGAGGATGCTTCGGGCGTGGCCGCGGTCTAAGTCCCCTGGAACGGGGCGTCGCAGAGGGTGAGAATCCCGTCTTCGGCCGGCCGGCCCCGCCCGTGTGAAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGTGGTAAATTTCATCTAAAGCTAAATACTGGTCGGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAACAGCATGTGAAATTGTTGAAAGGGAAGCGCTTGCGACCAGAGTCGGCCGCGGGGGCTCAGCGGGCACTTGTTGCCCGTGCACTCCCCCGTGGTCGGGCCAGCGTCGGTTTCGACGGCCGGTCAAAGGCCCCCGGAATGTGTCGCCTCTCGGGGCGTCTTATAGCCGGGGGTGCAATGCGGCCAGTCGGGACCGAGGAACGCGCTCCGGCACGGACGCTGGCTTAATGGTTGTAAGCGA

Verification method
Whole-genome Sequencing

History

Deposited as
Paracoccidioides brasiliensis (Splendore) Almeida
Depositors
CM Soares
Chain of custody
ATCC <-- CM Soares <-- M.R.R. Silva 01
Special collection
NCRR Contract
Cross references
GenBank AF148715 glucan synthase (FKS) gene, complete coding sequence
GenBank U91560 heat shock protein 70 gene, hsp70, complete coding sequence
GenBank ABKH00000000 Paracoccidioides lutzii Pb01, whole genome shotgun sequencing project

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Teixeira Mde M, et al. Paracoccidioides lutzii sp. nov.: biological and clinical implications. Med Mycol 52: 19-28, 2014. PubMed: 23768243

Paes HC, et al. Characterisation of the heat shock factor of the human thermodimorphic pathogen Paracoccidioides lutzii. Fungal Genet Biol 48: 947-955, 2011. PubMed: 21708278

Desjardins CA, et al. Comparative genomic analysis of human fungal pathogens causing paracoccidioidomycosis. PLoS Genet 7: e1002345, 2011. PubMed: 22046142

Glenn AE, et al. Comparative genomic and phylogenetic investigation of the xenobiotic metabolizing arylamine N-acetyltransferase enzyme family. FEBS Lett 584: 3158-3164, 2010. PubMed: 20621844

Teixeira MM, et al. Phylogenetic analysis reveals a high level of speciation in the Paracoccidioides genus. Mol Phylogenet Evol 52: 273-283, 2009. PubMed: 19376249

View All Curated Citations for this Product

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